Mark Stephen Greeley, Jr.

Biological Monitoring Group
Environmental Sciences Division
Oak Ridge National Laboratory
Oak Ridge, TN 37831-6038
Phone: (865) 574-9387
Fax: (865) 576-8646
greeleyms@ornl.gov

Professional Interests

Reproductive and developmental toxicology, aquatic ecology, biomarkers of contaminant exposure and effects, methods of environmental assessment.

Education

Ph.D., Biology, 1983, Univ. of Alabama at Birmingham
B.S., Biology - University of Alabama at Birmingham, 1978

Employment History

1991-present: Research Staff Member, Environmental Sciences Division, Oak Ridge National Laboratory (ORNL)
1988-1991: Scientist II, Automated Sciences Group, Inc. (on assignment at ORNL)
1983-1988: Postdoctoral Research Associate, Whitney Marine Laboratory, University of Florida
1978-1983 Graduate Teaching and Research Fellow, University of Alabama at Birmingham
1974-1978: Undergraduate Laboratory Instructor, University of Alabama at Birmingham

Qualifications/Experience

  • Over ten years experience managing ORNL Environmental Toxicology Laboratory
  • Aquatic Biologist with over 30 years of Freshwater and Estuarine Research Experience
  • Project Management, including Budget and Financial Analyses
  • Environmental Effects Assessments and Monitoring
  • Experience in numerous EPA and NIEHS Review Panels and Site Visits
  • Participation in National Academy of Sciences Committee

Select Publications and Reports

  • Southworth, G. R., S. C. Brooks, M.S. Greeley, Jr., R. H. Ketelle, and M.J. Peterson. In review. Sources of mercury to a contaminated stream – implications for time scale of effective remediation. Science of the Total Environment
  • Greeley, M., L. Kszos, G. Morris, J. Smith and A. Stewart. In review. Role of a comprehensive toxicity assessment and monitoring program in the management and ecological recovery of a wastewater receiving stream. Environmental Management.
  • Southworth, G., M. Greeley, M. Peterson., K. Lowe, and R. Ketelle (2010). Sources of mercury to East Fork Poplar Creek downstream from the Y-12 National Security Complex: Inventories and export rates. ORNL/TM-2009/231. Oak Ridge, TN. 69 pp.
  • Alexander, S., et al. (2010). Assessing ecotoxicological effects of fly ash at the Kingston TN Site: A white paper addressing data gaps and requirements. Report on the Ecotoxicological Working Group at the TVA-Kingston Fly Ash Release Environmental Research Symposium, March 11-12, 2010. TVA/ORAU.
  • National Research Council. (2005). Understanding Oil Spill Dispersants: Efficacy and Effects. The National Academies Press. Washington, D.C. 377 pp.
  • Adams, S.M., M.S. Greeley, D.G. Fitzgerald, J.M. Law, E.J. Noga, and J.T. Zelikoff (2004). Effects of flooding from three sequential hurricanes on the health and condition of fish in Pamlico Sound, NC. Estuaries 26: 1365-1382.
  • Hoyt, P. R., M. J. Doktycz, K. L. Beattie, and M. S. Greeley, Jr. (2003). DNA microarrays detect 4-nonylphenol-induced alterations in gene expression during zebrafish early development. Ecotoxicology 12: 469-474.
  • Schrader, E. A., T. R. Henry, M. S. Greeley, Jr., and B. P. Bradley (2003). Proteomics in zebrafish exposed to endocrine disrupting chemicals. Ecotoxicology12: 485-488.
  • Greeley, M.S., Jr. (2002). Reproductive Indicators of Environmental Stress in Fish. Pp. 321-377 in S. M. Adams (ed), Biological Indicators of Stress. American Fisheries Society, Bethesda, Maryland.
  • McMaster, M.E., J. J. Jardine, G. Ankley, W. H. Benson, M. Greeley, T. Gross, L. Guilette, D. MacLatchy, G. Van Der Kraak and K. R. Munkittrick. (2001). An interlaboratory study on the use of steroid hormones in examining endocrine disruption. Environ. Toxicol. Chem. 20: 2081 -2087.
  • Adams, S.M. and M.S. Greeley. (2000). Ecotoxicological indicators of water quality: Using multi-response indicators to assess the health of aquatic ecosystems. J. of Water, Air, and Soil Pollution 123:103-115.
  • Adams, S.M. and M.S. Greeley. (2000). Environmental challenges of water-quality assessment using integrated bioindicators to evaluate aquatic ecosystem health. Pages 103- 115 in S. Belkin (ed), Environmental challenges. Kluwer Academic Pubs., London.
  • Adams, S.M., M.S. Greeley, and M.G. Ryon. (2000). Evaluating effects of stressors on fish health at multiple levels of biological organization: Extrapolation from lower to higher levels. Human & Ecological Risk Assessment 6: 15-27.
  • Adams, S.M., M.S. Bevelhimer, M.S. Greeley. D.A. Levine, and S.J. Teh. (1999). Ecological risk assessment in a large river-reservoir: 6. Bioindicators of fish population health. Environ. Toxicol. Chem. 18:628-640.
  • Greeley, M.S., Jr., T.L. Ashwood, L.A. Kszos, M.J. Peterson, T.L. Phipps, C.D. Rash and G.R. Southworth. (1998). Postremediation Monitoring Program Baseline Assessment Report, Lower East Fork Poplar Creek (Y/ER-319). Bechtel Jacobs Company LLC, Oak Ridge, TN.
  • Garcia, E. F., R. McPherson, T.H. Martin, R. A. Poth and M.S. Greeley, Jr. (1997). Liver cell estrogen receptor binding in prespawning female largemouth bass, Micropterus salmoides, environmentally exposed to polychlorinated biphenyls (PCBs). Arch. Environm. Contam. Toxicol. 22: 309-315.
  • Adams, S. M., K.D. Ham, M.S. Greeley, Jr., R.F. LeHew, D.E. Hinton, and C.F. Saylor. (1996). Downstream gradients in bioindicator responses: Point source contaminant effects on fish health. Can. J. Fish. Aquat. Sci. 53: 2177-2187.
  • Hsiao, S.-M., M. S. Greeley, and R. A. Wallace. (1994). Reproductive cycling in female Fundulus heteroclitus. Biological Bulletin 186: 271-284.
  • Adams, S.M., and M.S. Greeley, Jr. (1996) Establishing possible links and relationships between aquatic ecosystem health and human health: An integrated approach. Pages 91-102 in R.T. DiGiulio and E. Monosson (eds.), Interconnections between Human and Ecosystem Health. Chapman and Hall, Publ.
  • Greeley, M.S., Jr., S.M. Adams, W.D. Crumby, R. Epler, D.L. Harris, K.L. Lee, M.K. McCracken, J.G. Mural, S.L. Niemela, R. McPherson, D.E. Hinton and R. Stripp. (1994). Bioindicator Assessment of Fish Health and Reproductive Success in Lake Hartwell and Twelve Mile Creek. In Biological Investigation for the Remedial Investigation/Feasibility Study, Sangamo Weston, Inc./Twelve Mile Creek/Lake Hartwell PCB Contamination Superfund Site. U.S. Army Corps of Engineers, Savannah.
  • Adams, S.M., G.F. Cada, M.S. Greeley, Jr., and L.R. Shugart. (1993). Evaluating effects of environmental stress on fish communities using multi-response indicators, pp. 170-177. In Environmental Analysis: The NEPA Experience (S.G. Hildebrand and J.B. Cannon, eds.). Lewis Publ., Boca Raton, FL.
  • Adams, S.M., W.D. Crumby, M.S. Greeley, Jr., and L.R. Shugart (1992). Responses of fish populations and communities to pulp mill effluents: A holistic assessment. Ecotoxicol. Environmental Safety 24: 347-360.
  • Adams, S. M., W.D. Crumby, M.S. Greeley, Jr., M.G. Ryon, and E.M. Schilling (1992). Relationship between physiological and fish population responses in a contaminated stream. Environ. Toxicol. Chem. 11: 1549-1557.
  • Wallace, R.A., M.S. Jr. Greeley, and R. McPherson (1992). Analytical and experimental studies on the relationship between Na+, K+, and water uptake during volume increases associated with Fundulus oocyte maturation in vitro. Journal of Comparative Physiology 162B: 241-248.
  • Shugart, L. R., S. J. D’Surney, C. Getty-Hull, and M. S. Greeley, Jr. (1991). Biological (Molecular and Cellular) Markers of Toxicity: Final Report to the U. S. Army Biomedical Research and Development Laboratory (ORNL/M-1845). Oak Ridge National Laboratory. Oak Ridge, TN.
  • Adams, S. M., and M.S. Greeley, Jr. (1991). Assessment and evaluation of the ecological health of fish populations exposed to PCBs in Hartwell Reservoir. Tennessee Valley Authority Publ. TVA/WR/AB-91/14, Chattanooga, TN.
  • Greeley, M.S., Jr., H. Hols, and R.A. Wallace (1991). Changes in size, hydration, and low molecular weight osmotic effectors during meiotic maturation of Fundulus oocytes in vivo. Comp. Biochem. Physiol. 100A: 639-647.
  • McPherson, R., M. S. Greeley, Jr., and R. A. Wallace (1989). The influence of yolk protein proteolysis on hydration in the oocytes of Fundulus heteroclitus. Development, Growth and Differentiation 31: 475-483.
  • Adams, S.M., K.L. Shepard, M.S. Greeley, Jr., B.D. Jimenez, M.G. Ryon, L.R. Shugart, J.F. McCarthy, and D.E. Hinton (1989). The use of bioindicators for assessing the effects of pollutant stress on fish. Mar. Environ. Res. 28: 459-464.
  • Petrino, T. R., M. S. Greeley, Jr., K. Selman, Y-W. P. Lin, and R. A. Wallace (1989). Steroidogenesis in Fundulus heteroclitus II. Production of 17α-hydroxy, 20ß-dihydroprogesterone, testosterone, and 17β-estradiol by various isolated components of the ovarian follicle. Gen. Comp. Endocrinol. 76: 230-240.
  • Lin, Y-W. P., M. S. Greeley, Jr., and R. A. Wallace (1989). Fundulus heteroclitus gonadotropin(s) 2. Year-round husbandry of animals with active pituitaries and responsive follicles. Fish Physiol. Biochem. 6: 139-148.
  • Greeley, M. S., Jr., R. MacGregor III, and K. R. Marion (1988). Variation in plasma estrogens of the gulf killifish (Fundulus grandis Baird and Girard). J. Fish Biol. 33: 419-429.
  • Greeley, M. S., Jr., D. R. Calder, and R. A. Wallace (1987). Oocyte growth and development during seasonal ovarian recrudescence in the striped mullet (Mugil cephalus): Relationship to fecundity and age at maturity. Fishery Bull. 85: 187-200.

Synergistic Activities

  • Principal Investigator, Fish reproduction and larval success research projects related to the TVA Kingston (TN) Steam Plant Coal Ash Spill (2009-present)
  • Editorial Board, Journal Ecotoxicology (2004 – present)
  • Manager, Environmental Toxicology Laboratory, Oak Ridge National Laboratory, (1999–present)
  • Project Manager, Biological Monitoring and Abatement Program, NNSA/DOE Oak Ridge Y-12 National Security Complex (1996–2006)
  • Member of National Academies Committee on Understanding Oil Spill Dispersants: Effects and Efficacy (2004-2005)
  • Project Manager, DOE Lower East Fork Poplar Creek Postremediation Monitoring Program (1996–1998)
  • ORNL Project Manager, DOD Ashumet and Johns Ponds Investigation, Massachusetts Military Reservation (1993–1996)
  • ORNL Project Manager, Biological Indicators Investigation, EPA/US Army Corps of Engineers, Sangamo Weston Inc./Hartwell Reservoir PCB Contamination Remedial Investigation (1992-1995)
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